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๐ฌ ๐งช Two useful approaches: ๐น Bradford Assay โ ProteinโCoomassie binding โ Read at ~595 nm ๐น BCA Assay โ Cuยฒโบ reduction + BCA complex โ Read at ~562 nm ๐ Donโt forget the complete QC check: โ A260/A280 โ A260/A230 โ Qubit/fluorometric concentration โ Bradford/BCA for residual protein โ Agarose gel / RNA integrity assessment โ ๏ธ Important: Extraction reagents can interfere with Bradford/BCA assays
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