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Methods Sample preparation for ice nucleation experiments Aqueous solutions were prepared at concentrations of 5 mg mL −1 , 0.5 mg mL −1 , and 5 × 10 −4 mg mL −1 using ultrapure water (HPLC water, Sigma-Aldrich) and commercially available compounds, which were obtained from Sigma-Aldrich unless otherwise noted

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On one hand, cuproplasia, the term that refers to copper-driven cellular growth and proliferation, involves multiple cancer pathways and regulatory mechanisms to promote tumor progression, 17 which could be further accelerated by copper-dependent metastasis, angiogenesis, and immune escape